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Article RSE 14008309

Enzymatic Oxygen Micro-sensor based on Bilirubin Oxidase applied to Microbial Fuel Cells analysis

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Electroanalysis Journal DOI: 10.1002/elan.201400543, pp. 327-335, Gennaio-2015.

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C. Santoro (University of New Mexico), P. Cristiani (RSE SpA), M. Bestetti (Politecnico di Milano), P. Atanassov (University of New Mexico), E. Guerrini (Università degli studi di Milano), S. PM Trasatti (Università degli studi di Milano), M. Grattieri (Politecnico di Milano), S. Babanova (University of New Mexico)

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A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater.

A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3 mg L-1 of O2 in the bulk solution, decreasing to 0 mg L-1 in the cathodic biofilm. The MFC generated power in the range of 0 – 0.08 mW, associated to the oxygen content.

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