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DGGE profiles of biofilms operating in membraneless Microbial Fuel Cells on different anode materials

pubblicazioni - Poster

DGGE profiles of biofilms operating in membraneless Microbial Fuel Cells on different anode materials

The microbial communities at the anode, made of different materials, of one month operating membraneless microbial fuel cells has been analyzed by the fingerprinting molecular technique, DGGE (Denaturing gradient gel electrophoresis). The anode materials sampled were: carbon fibres of a brush, carbon cloth and stainless steel chips (AISI 304). The microbial fuel cells were inoculated with row wastewater coming from a municipal plant of Milan city and weekly fed with sodium acetate 3 g/l. DGGE profiles enabled to calculate the Jaccard similarity indexes for the bacterial communities that developed on the anode of the MFC. In this way the degree of selection of microbial communities was defined in relation to the constructive characteristics and materials used. The cluster analysis based on similarity data, showed that the microbial populations, coming from the same inoculum, gradually diverged in relation to the structural characteristics of the cell, and in particular to the material and the geometry of the anode. In addition, the excision and sequencing of some bands shared by all the anodic communities showed the presence of bacteria belonging to the group of Spirochaete.

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