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Enzymatic Oxygen Micro-sensor based on Bilirubin Oxidase applied to Microbial Fuel Cells analysis

pubblicazioni - Articolo

Enzymatic Oxygen Micro-sensor based on Bilirubin Oxidase applied to Microbial Fuel Cells analysis

In questo lavoro è stato sviluppato e validato un biosensore di ossigeno selettivo basato sull’enzima bilirubina ossidasi. Il biosensore è stato quindi utilizzato per misurare i profili di ossigeno tra anodo e catodo di una cella a combustibile microbica a singola camera, alimentata con acqua reflua.

A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater.

The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3 mg L-1 of O2 in the bulk solution, decreasing to 0 mg L-1 in the cathodic biofilm. The MFC generated power in the range of 0 – 0.08 mW, associated to the oxygen content.

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